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Literature summary extracted from

  • Knipp, M.; Yang, F.; Berry, R.E.; Zhang, H.; Shokhirev, M.N.; Walker, F.A.
    Spectroscopic and functional characterization of nitrophorin 7 from the blood-feeding insect Rhodnius prolixus reveals an important role of its isoform-specific N-terminus for proper protein function (2007), Biochemistry, 46, 13254-13268.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.6.1 expression in Escherfichia coli Rhodnius prolixus

Protein Variants

EC Number Protein Variants Comment Organism
1.7.6.1 additional information truncated mutant enzyme NP7(DELTA1-3) lacking the 0Met-Leu-Pro-Gly3 sequence, shows marked decay above 45°C. Wild-type nitrophorin 7, in contrast, is comparatively stable, and does not experience a marked signal decrease of activity at temperatures below 52°C Rhodnius prolixus

General Stability

EC Number General Stability Organism
1.7.6.1 the N-terminus of NP7 significantly stabilizes the protein fold Rhodnius prolixus

Organism

EC Number Organism UniProt Comment Textmining
1.7.6.1 Rhodnius prolixus Q6PQK2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.6.1 saliva
-
 Rhodnius prolixus
-

Synonyms

EC Number Synonyms Comment Organism
1.7.6.1 Nitrophorin 7
-
 Rhodnius prolixus
1.7.6.1 NP7
-
 Rhodnius prolixus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.6.1 45
-
 truncated mutant enzyme NP7(DELTA1-3) shows marked decay above 45°C. Wild-type nitrophorin 7, in contrast, is comparatively stable, and does not experience a marked signal decrease of activity at temperatures below 52 °C Rhodnius prolixus
1.7.6.1 52
-
 truncated mutant enzyme NP7(DELTA1-3) shows marked decay above 45°C. Wild-type nitrophorin 7, in contrast, is comparatively stable, and does not experience a marked signal decrease of activity at temperatures below 52 °C Rhodnius prolixus

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.6.1 heme nitrophorin 7 strongly favors the A orientation of the heme Rhodnius prolixus